Review



recombinant canine ifn γ  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    R&D Systems recombinant canine ifn γ
    Recombinant Canine Ifn γ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+canine+ifn+%CE%B3/Recombinant+Canine+IFN-gamma+Protein/pmc13129499-59-0-8
    Average 93 stars, based on 22 article reviews
    recombinant canine ifn γ - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Pathogenic T cell responses against aquaporin 4
    Article Snippet: .. Madin–Darby canine kidney (MDCK) cells were cultured in the presence or absence of 50 ng/ml recombinant canine IFN-γ (R&D Systems) for the last 72 h. Then, the cells were detached with accutase (PAA Laboratories GmbH, Pansching, Austria) and incubated for 30 min at 4°C with human NMO-IgG (stock 10 mg/ml) or with Subcuvia as human control IgG. .. All antibody preparations were used at a dilution of 1:100 in stain buffer (BD Pharmingen, San Diego, USA).

    Recombinant:

    Article Title: Pathogenic T cell responses against aquaporin 4
    Article Snippet: .. Madin–Darby canine kidney (MDCK) cells were cultured in the presence or absence of 50 ng/ml recombinant canine IFN-γ (R&D Systems) for the last 72 h. Then, the cells were detached with accutase (PAA Laboratories GmbH, Pansching, Austria) and incubated for 30 min at 4°C with human NMO-IgG (stock 10 mg/ml) or with Subcuvia as human control IgG. .. All antibody preparations were used at a dilution of 1:100 in stain buffer (BD Pharmingen, San Diego, USA).

    Article Title: Characterization of polarization states of canine monocyte derived macrophages
    Article Snippet: .. At day 5, GM-CSF treated monocytes were activated with 20 ng/ml recombinant canine IFN-γ (R&D system, Abingdon, United Kingdom) and 100 ng/ml LPS from Escherichia coli O111:B4 (Sigma-Aldrich, Saint Louis, MO, USA) for M1 polarization; M-CSF treated monocytes were activated with 40 ng/ml recombinant canine IL-4 (R&D system, Abingdon, United Kingdom) for M2 polarization. ..

    Article Title: Toll-like receptor 2 activation induces C-C motif chemokine ligand 5 production in canine keratinocytes.
    Article Snippet: Canine atopic dermatitis (cAD) is a chronic recurrent cutaneous inflammatory disease.. In the skin of dogs with atopic dermatitis (AD), the overgrowth of Staphylococcus spp., especially S. pseudintermedius, is frequently detected.. Combined antimicrobial and antiinflammatory (oclacitinib or glucocorticoids) treatment decreased the population of Staphylococcus spp. on the skin and improved the severity of skin lesions in dogs with AD.

    Article Title: Tumour necrosis factor-α induces C-C motif chemokine ligand 5 production in canine keratinocytes.
    Article Snippet: Canine atopic dermatitis (cAD) is an allergic inflammatory disease characterised by the infiltration of activated immune cells, including T cells, eosinophils and mast cells, into the skin.. In addition to the activation of Thelper (Th)2type cells, Th1type cells have been shown to play an important role in the pathogenesis of cAD, particularly in the chronic phase of the disease.. CC motif chemokine ligand (CCL)5, also known as RANTES (regulated on activation, normal Tcell expressed and secreted), is a chemokine produced by CD8+T cells, epithelial cells and fibroblasts.


    Article Title: Characterization of polarization states of canine monocyte derived macrophages.
    Article Snippet: .. At day 5, GM-CSF treated monocytes were activated with 20 ng/ml recombinant canine IFN-γ (R&D system, Abingdon, United Kingdom) and 100 ng/ml LPS from Escherichia coli O111:B4 (Sigma-Aldrich, Saint Louis, MO, USA) for M1 polarization; M-CSF treated monocytes were activated with 40 ng/ml recombinant canine IL-4 (R&D system, Abingdon, United Kingdom) for M2 polarization. ..

    Article Title: Immune regulation of canine tumour and macrophage PD-L1 expression.
    Article Snippet: Expression of programmed cell death receptor ligand 1 (PD-L1) on tumor cells has been associated with immune escape in human and murine cancers, but little is known regarding the immune regulation of PD-L1 expression by tumor cells and tumor-infiltrating macrophages in dogs.. Therefore, 14 canine tumor cell lines, as well as primary cultures of canine monocytes and macrophages, were evaluated for constitutive PD-L1 expression and for responsiveness to immune stimuli.. We found that PD-L1 was expressed constitutively on all canine tumor cell lines evaluated, although the levels of basal expression were very variable.

    Incubation:

    Article Title: Pathogenic T cell responses against aquaporin 4
    Article Snippet: .. Madin–Darby canine kidney (MDCK) cells were cultured in the presence or absence of 50 ng/ml recombinant canine IFN-γ (R&D Systems) for the last 72 h. Then, the cells were detached with accutase (PAA Laboratories GmbH, Pansching, Austria) and incubated for 30 min at 4°C with human NMO-IgG (stock 10 mg/ml) or with Subcuvia as human control IgG. .. All antibody preparations were used at a dilution of 1:100 in stain buffer (BD Pharmingen, San Diego, USA).

    Article Title: Toll-like receptor 2 activation induces C-C motif chemokine ligand 5 production in canine keratinocytes.
    Article Snippet: Canine atopic dermatitis (cAD) is a chronic recurrent cutaneous inflammatory disease.. In the skin of dogs with atopic dermatitis (AD), the overgrowth of Staphylococcus spp., especially S. pseudintermedius, is frequently detected.. Combined antimicrobial and antiinflammatory (oclacitinib or glucocorticoids) treatment decreased the population of Staphylococcus spp. on the skin and improved the severity of skin lesions in dogs with AD.

    Article Title: Tumour necrosis factor-α induces C-C motif chemokine ligand 5 production in canine keratinocytes.
    Article Snippet: Canine atopic dermatitis (cAD) is an allergic inflammatory disease characterised by the infiltration of activated immune cells, including T cells, eosinophils and mast cells, into the skin.. In addition to the activation of Thelper (Th)2type cells, Th1type cells have been shown to play an important role in the pathogenesis of cAD, particularly in the chronic phase of the disease.. CC motif chemokine ligand (CCL)5, also known as RANTES (regulated on activation, normal Tcell expressed and secreted), is a chemokine produced by CD8+T cells, epithelial cells and fibroblasts.

    Control:

    Article Title: Pathogenic T cell responses against aquaporin 4
    Article Snippet: .. Madin–Darby canine kidney (MDCK) cells were cultured in the presence or absence of 50 ng/ml recombinant canine IFN-γ (R&D Systems) for the last 72 h. Then, the cells were detached with accutase (PAA Laboratories GmbH, Pansching, Austria) and incubated for 30 min at 4°C with human NMO-IgG (stock 10 mg/ml) or with Subcuvia as human control IgG. .. All antibody preparations were used at a dilution of 1:100 in stain buffer (BD Pharmingen, San Diego, USA).



    Similar Products

    93
    R&D Systems recombinant canine ifn γ
    Recombinant Canine Ifn γ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+canine+ifn+%CE%B3/Recombinant+Canine+IFN-gamma+Protein/pmc13129499-59-0-8
    Average 93 stars, based on 1 article reviews
    recombinant canine ifn γ - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems recombinant interferon γ
    Recombinant Interferon γ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+canine+ifn+%CE%B3/Recombinant+Canine+IFN-gamma+Protein/pm40341986-59-35-40
    Average 93 stars, based on 1 article reviews
    recombinant interferon γ - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems recombinant interferon ɣ
    Recombinant Interferon ɣ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+canine+ifn+%CE%B3/Recombinant+Canine+IFN-gamma+Protein/pm40341986-126-77-79
    Average 93 stars, based on 1 article reviews
    recombinant interferon ɣ - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems canine ifn γ
    Canine Ifn γ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+canine+ifn+%CE%B3/Recombinant+Canine+IFN-gamma+Protein/pm40200149-60-11-16
    Average 93 stars, based on 1 article reviews
    canine ifn γ - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems canine ifn
    Canine Ifn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+canine+ifn+%CE%B3/Recombinant+Canine+IFN-gamma+Protein/pm39860989-87-18-22
    Average 93 stars, based on 1 article reviews
    canine ifn - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    R&D Systems canine recombinant ifnγ
    Figure 6. The effect of pro-inflammatory stimulation on the synthesis of IDO1 in human and canine MSC(WJ)s. Western blot. (A,B) Representative blots presenting IDO1 protein expression in human (A) and canine (B) MSC(WJ)s treated for 24 h with different combinations of <t>IFNγ,</t> TNF, and poly(I:C). Yellow arrows indicate treatments chosen for extended analysis. (C–G) Representative blots presenting the effect of chosen treatments: IFNγ and IFNγ + poly(I:C) in human (C) and canine (D) MSC(WJ)s. (E,F) Mean (±SEM) optical density of IDO-1 normalized to β-actin (n = 10) in hMSC(WJ)s (E) and cMSC(WJ)s (F); 3 independent experiments, cells from 6 different donors in each species, n = 10, analyzed using Wilcoxon test, **, p < 0.01. (G) the comparison of the effect of both treatments on hMSC(WJ)s and cMSC(WJ)s. Mean (±SEM) fold change after treatment in comparison to untreated cells. Mann–Whitney U test, ns—statistically non-significant, *, p < 0.05.
    Canine Recombinant Ifnγ, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+canine+ifn+%CE%B3/Recombinant+Canine+IFN-gamma+Protein/pm39201612-461-32-35
    Average 93 stars, based on 1 article reviews
    canine recombinant ifnγ - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Figure 6. The effect of pro-inflammatory stimulation on the synthesis of IDO1 in human and canine MSC(WJ)s. Western blot. (A,B) Representative blots presenting IDO1 protein expression in human (A) and canine (B) MSC(WJ)s treated for 24 h with different combinations of IFNγ, TNF, and poly(I:C). Yellow arrows indicate treatments chosen for extended analysis. (C–G) Representative blots presenting the effect of chosen treatments: IFNγ and IFNγ + poly(I:C) in human (C) and canine (D) MSC(WJ)s. (E,F) Mean (±SEM) optical density of IDO-1 normalized to β-actin (n = 10) in hMSC(WJ)s (E) and cMSC(WJ)s (F); 3 independent experiments, cells from 6 different donors in each species, n = 10, analyzed using Wilcoxon test, **, p < 0.01. (G) the comparison of the effect of both treatments on hMSC(WJ)s and cMSC(WJ)s. Mean (±SEM) fold change after treatment in comparison to untreated cells. Mann–Whitney U test, ns—statistically non-significant, *, p < 0.05.

    Journal: International journal of molecular sciences

    Article Title: The Comparison of Immunomodulatory Properties of Canine and Human Wharton Jelly-Derived Mesenchymal Stromal Cells.

    doi: 10.3390/ijms25168926

    Figure Lengend Snippet: Figure 6. The effect of pro-inflammatory stimulation on the synthesis of IDO1 in human and canine MSC(WJ)s. Western blot. (A,B) Representative blots presenting IDO1 protein expression in human (A) and canine (B) MSC(WJ)s treated for 24 h with different combinations of IFNγ, TNF, and poly(I:C). Yellow arrows indicate treatments chosen for extended analysis. (C–G) Representative blots presenting the effect of chosen treatments: IFNγ and IFNγ + poly(I:C) in human (C) and canine (D) MSC(WJ)s. (E,F) Mean (±SEM) optical density of IDO-1 normalized to β-actin (n = 10) in hMSC(WJ)s (E) and cMSC(WJ)s (F); 3 independent experiments, cells from 6 different donors in each species, n = 10, analyzed using Wilcoxon test, **, p < 0.01. (G) the comparison of the effect of both treatments on hMSC(WJ)s and cMSC(WJ)s. Mean (±SEM) fold change after treatment in comparison to untreated cells. Mann–Whitney U test, ns—statistically non-significant, *, p < 0.05.

    Article Snippet: IFNγ and TNF were used in a species-specific manner—human recombinant IFNγ (R&D System, Minneapolis, MN, USA, Cat#: 285-IF) and human recombinant TNF (Peprotech, Cheshire UK, Cat#: 300-01A) were added to hMSC(WJ)s, whereas canine recombinant IFNγ (R&D System, Cat#: 781-CG) and canine recombinant TNF (R&D System, Cat#: 1507-CT) were added to cMSC(WJ)s; all at a concentration of 20 ng/mL.

    Techniques: Western Blot, Expressing, Comparison, MANN-WHITNEY

    Figure 7. Transforming growth factor beta 1 (TGFβ1) secretion by MSC(WJ)s. ELISA. Data blanked to medium alone. (A) basal secretion of TGFβ1 by unstimulated human and canine MSC(WJ)s. (B,C) the effect of pro-inflammatory stimulation on TGFβ1 secretion by human (B) and canine (C) cells. (D) comparison of response to recombinant (species-matched) IFNγ (20 ng/mL) on TGFβ1 secretion between human and canine MSC(WJ)s. The graph presents mean delta values (TGFβ1IFNγ— TGFβ1CTRL). ns—statistically not significant, *, p < 0.05; n = 6 for each cell type, assay performed in duplicates.

    Journal: International journal of molecular sciences

    Article Title: The Comparison of Immunomodulatory Properties of Canine and Human Wharton Jelly-Derived Mesenchymal Stromal Cells.

    doi: 10.3390/ijms25168926

    Figure Lengend Snippet: Figure 7. Transforming growth factor beta 1 (TGFβ1) secretion by MSC(WJ)s. ELISA. Data blanked to medium alone. (A) basal secretion of TGFβ1 by unstimulated human and canine MSC(WJ)s. (B,C) the effect of pro-inflammatory stimulation on TGFβ1 secretion by human (B) and canine (C) cells. (D) comparison of response to recombinant (species-matched) IFNγ (20 ng/mL) on TGFβ1 secretion between human and canine MSC(WJ)s. The graph presents mean delta values (TGFβ1IFNγ— TGFβ1CTRL). ns—statistically not significant, *, p < 0.05; n = 6 for each cell type, assay performed in duplicates.

    Article Snippet: IFNγ and TNF were used in a species-specific manner—human recombinant IFNγ (R&D System, Minneapolis, MN, USA, Cat#: 285-IF) and human recombinant TNF (Peprotech, Cheshire UK, Cat#: 300-01A) were added to hMSC(WJ)s, whereas canine recombinant IFNγ (R&D System, Cat#: 781-CG) and canine recombinant TNF (R&D System, Cat#: 1507-CT) were added to cMSC(WJ)s; all at a concentration of 20 ng/mL.

    Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Recombinant